This second edition of Instrumental Methods of Analysis is tailored for Semester VII B. Pharmacy students under the revised Credit System Syllabus of DBATU, Lonere (effective 2018-2019). The book offers a concise and simplified explanation of complex pharmaceutical analysis concepts, making it student-friendly. Covering five comprehensive units, it details the principles, instrumentation, techniques, and applications of key analytical methods. Developed using authoritative references, recent journal insights, and the authors’ experience, the content aligns closely with syllabus requirements and lecture hours. It serves as a practical and accessible guide for both students and educators, with room for future enhancements through feedback.